SpheroMetrics
See the Invasion. Measure the Future.
One platform · Three signals · Zero uploads
Every spheroid, decomposed.
Spheroid analysis
has been painful.
Manual ImageJ thresholding. $60k benchtop instruments. Server uploads that violate IRB. None of it had to be this way.
The old way
- ✕Manual threshold tuning per image series
- ✕$60,000+ capital equipment (Cytation 5, Incucyte)
- ✕Server uploads · IT compliance nightmares
- ✕No project management across 96-well plates
SpheroMetrics
- ✓Adjust sliders, watch overlays redraw live
- ✓Free · Open · runs in any modern browser
- ✓All processing local. Nothing uploaded. Ever.
- ✓Native 96-well projects, conditions, replicates
One platform. Two pipelines.
Every spheroid imaged.
Scroll to explore each pipeline. Switch between fluorescence-aware invasion analysis and label-free morphometry without ever leaving the browser.
GFP Invasion Assay
Dual-channel segmentation isolates GFP-positive tumor core, magenta invasion zone, and the total spheroid body — all from a single image.
Brightfield Area Assay
Label-free spheroid segmentation using Gaussian background estimation and connected-component analysis — perfect for time-course studies.
GE = min(G − R, G − B) · Gaussian background subtraction · Connected components · Sub-second per image
A whole experiment.
One living plate.
Organize conditions, replicates, and time points across an entire 96-well experiment. Every spheroid measurement, stored alongside its well.
Cross-validated against
ImageJ & Cytation 5.
High concordance with the industry standards — without the price tag, server uploads, or installation hassle.
| Tool | GFP Invasion | Brightfield | No Install | Client-side | 96-Well Mgmt | Free / Open |
|---|---|---|---|---|---|---|
SpheroMetrics6/6 | ||||||
SpheroScan3/6 | — | — | — | |||
AnaSP3/6 | — | — | — | |||
ImageJ3/6 | — | — | — | |||
Incucyte®3/6 | — | — | — | |||
Cytation 54/6 | — | — |
Everything a spheroid lab needs.
Nothing it doesn't.
Dual-channel GFP
Simultaneous GFP core, invasion zone, and total body segmentation from one image.
Brightfield mode
Label-free spheroid area, diameter, circularity, perimeter — Cytation 5 compatible.
Freehand ROI
Lasso any region; segmentation runs only inside your selection. Debris excluded.
Live overlays
Adjust thresholds and watch masks redraw in real time. No re-processing wait.
96-well project mgmt
Conditions, replicates, time points — organized across every well, every plate.
Excel export
Raw + summary + per-project + heatmap sheets. Ready for stats software.
The algorithms,
in the open.
No black boxes. Every parameter exposed. Every step inspectable. Reproducibility is the default, not a feature.
Per-pixel green-channel dominance over red and blue identifies true GFP signal — robust to autofluorescence and uneven illumination.
A configurable Gaussian blur estimates local background brightness; subtracting it isolates true spheroid darkness from imaging artefacts.
Pixels meeting threshold are grouped into connected regions; only components above the minimum area survive. Noise, gone.
Researchers who switched.
And never went back.
The first spheroid tool I haven't had to fight with. I dropped 96 wells in and had publication-ready measurements before my coffee finished brewing.
We run invasion assays every week, and quantifying the GFP halo around each spheroid used to eat an entire afternoon in ImageJ. With SpheroMetrics I batch a full 96-well plate in minutes and the invasion-index numbers line up with what I'd measure by hand.
The animations on the segmentation overlays are actually insane. But what got my lab to switch is the Excel export. It just works.
Start analyzing in your browser.
Zero install. Zero risk.
Free · Open · v5.0 · Made for the bench